Metabolite Profiling of Temelekar Root (Coptosapleta tementosa Valeton ex. K. Heyne) Using Chemometric Methods

Abstract

Coptosapelta tementosa Valeton ex. K. Heyne is known to contain steroid, flavonoid and phenolic compounds. The entity and content of bioactive compounds of plants are  depending on the type of soil and climate of its grown place, which can cause inconsistencies in terms of quality and efficacy. This study aims to determine the metabolite profiles of the ethanol extract of temelekar root, obtained from several growing places using FTIR and chemometric methods. Temelekar root samples were obtained from 4 different places on the island of Kalimantan (Kuaro, Babulu, Lintang Jaya, Tanjung Batu). Temelekar root was extracted using reflux method with 95% ethanol. The FTIR spectrum data were processed using a chemometric analysis program with statistical analysis data using the Unscramble 10 and Minitab version 19 programs. The results showed that the metabolite profiles of temelekar root from several grown places had significant differences. The similarity of variable area in Kuaro and Babulu (group 1) has a similarity of 85.09%. The variable area of Lintang Jaya with the variable area of group 1 has a similarity of 79.66% (group 2). The variable area of the Tanjung Batu and the variable area of ​​group 2 has 33.94% similarities (group 3). So, the conclusion is the grown place affect to production of secondary metabolite coumponds.