Activity Test and Formulation of Antioxidant Cream from Ethanol Extract Combination Of Several Citrus Fruit Peels (Citrus sp.)

Abstract

Peel lime fruit (Citrus aurantifolia Swingle.), kaffir lime (Citrus hystrix DC), sweet orange (Citrus sinensis L.) contains flavonoids and vitamin C which can ward off free radicals to prevent premature aging. This study aims to determine the free radical inhibitory activity of the ethanol extract of the skin of lime, kaffir lime, sweet orange, and formulate them in cream dosage forms. In this study, the peel extracts of lime, kaffir lime, and sweet orange were tested for free radical inhibition activity and formulated into cream preparations with various concentrations (tween 80-span 80) as much as (1%, 2%, 3%). The results showed that lime peel extract, can inhibit free radicals at a concentration (IC₅₀) 240 ppm, kaffir lime peel extract (IC₅₀) 210 ppm, sweet orange peel extract (IC₅₀) 133 ppm, and combination of extracts (IC₅₀) 67 ppm. The combination extract was then formulated in the form of a cream and the results showed that all cream formula met the stability requirements, and the optimum formula was formula I.